Integrative physiological, transcriptome, and metabolome analyses reveal the associated genes and metabolites involved in cold stress response in common vetch (<i>Vicia sativa</i> L.)

As an economically important legume, Vicia sativa is commonly used as fodder for livestock and in health-promoting foods human consumption; however, cold stress one of the most limitations to its utilization at high altitudes. The shoot tissues three cold-resistant (Lan1, Lan2, Lan3) cold-sensitive (368, 521, 538) accessions were collected after (4°C) treatment 0, 6, 48 h assess their molecular regulatory mechanisms during via integrative analyses transcriptomes metabolomes. Our Kyoto Encyclopedia Genes Genomes enrichment analysis showed that biosynthesis phenylpropanoids was enriched pathway response V. sativa. A total 18 differential metabolites obtained phenylpropanoid pathway, 722 differentially expressed genes (DEGs) a significant positive correlation with naringenin p-coumaric acid following weighted gene co-expression network analysis, while 84 DEGs negative associations. Moreover, four overexpressed yeast, it indicated biological function confer tolerance. These results provide valuable resource understanding underlying mechanism sativa's furthering development breeding research.